| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,237,438 | G→A | 24.5% | P461S (CCA→TCA) | ycgB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,237,438 | 0 | G | A | 24.5% | 90.8 / 28.5 | 49 | P461S (CCA→TCA) | ycgB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (24/13); new base A (9/3); total (33/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
CCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATAT > W3110S.gb/1237371‑1237497 | ccccACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGaaa > 1:3111914/1‑70 (MQ=255)ccccACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGaaa > 1:3182712/1‑70 (MQ=255) cccACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAata < 1:3242203/71‑1 (MQ=255) ccACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAatat < 1:3043460/71‑1 (MQ=255) ccACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAatat < 1:4350523/71‑1 (MQ=255) ccACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAatat < 1:637103/71‑1 (MQ=255) ccACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAatat < 1:2178892/71‑1 (MQ=255) caGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAAc > 1:1146805/1‑71 (MQ=255) caGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAAc > 1:3225830/1‑71 (MQ=255) caGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAAc > 1:730161/1‑71 (MQ=255) gATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGc > 1:3047434/1‑71 (MQ=255) gCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGt > 1:4751258/1‑70 (MQ=255) cacaTGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTc < 1:911214/70‑1 (MQ=255) cacaTGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTc < 1:1439113/70‑1 (MQ=255) cacaTGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAat > 1:2904124/1‑57 (MQ=255) cacaTGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTc < 1:4148464/70‑1 (MQ=255) aTGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCg < 1:2499067/71‑1 (MQ=255) tGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGa > 1:707524/1‑51 (MQ=255) tGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCg > 1:1760346/1‑70 (MQ=255) gCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGaa > 1:2653027/1‑71 (MQ=255) ttCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATaa > 1:3238689/1‑54 (MQ=255) cAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACgg > 1:4808907/1‑70 (MQ=255) ggACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGaa > 1:4724935/1‑65 (MQ=255) ggACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGaa > 1:3432141/1‑65 (MQ=255) ggACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAAAGCAGCGACAGCGaa > 1:204012/1‑65 (MQ=255) ttcttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGaa > 1:369578/1‑41 (MQ=255) ttcttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGaa > 1:3766446/1‑41 (MQ=255) cttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCcgcg > 1:4150383/1‑70 (MQ=255) cttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCcgcg > 1:2134846/1‑70 (MQ=255) cttTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCcgcg > 1:1726444/1‑70 (MQ=255) tGCGCCCCCGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCaa < 1:1069648/70‑1 (MQ=255) cGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCaaa > 1:4819115/1‑63 (MQ=255) cGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGtt > 1:4126497/1‑71 (MQ=255) cGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGtt > 1:1058804/1‑71 (MQ=255) cGATCCAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGtt > 1:4262759/1‑71 (MQ=255) cAGCGGTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGa < 1:4050919/71‑1 (MQ=255) aGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTc > 1:3195740/1‑66 (MQ=255) cGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCt < 1:379859/71‑1 (MQ=255) ggTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCt > 1:4401166/1‑70 (MQ=255) ggTGCGCGATTATGTGAAATATAACGCAGCGTCAGCGAACGGTcgc > 1:911713/1‑46 (MQ=255) gTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCa > 1:1251788/1‑64 (MQ=255) gTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCa > 1:3081629/1‑64 (MQ=255) gcgcGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTc < 1:2229251/60‑1 (MQ=255) gcgcGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTc < 1:1934720/60‑1 (MQ=255) cgcgATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAata < 1:2225988/71‑1 (MQ=255) gcgATTATGTGGAATATAACGCAGCGTCAGCGAACg < 1:4155991/36‑1 (MQ=255) gcgATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAatat > 1:436093/1‑71 (MQ=255) gcgATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAatat > 1:4893909/1‑71 (MQ=255) gcgATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAatat > 1:152409/1‑71 (MQ=255) cgATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAatat > 1:2877686/1‑70 (MQ=255) aTTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAata > 1:2704355/1‑67 (MQ=255) | CCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATAT > W3110S.gb/1237371‑1237497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |