| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,254,608 | T→C | 25.0% | intergenic (+46/+54) | dhaR → / ← ycgV | predicted DNA‑binding transcriptional regulator, dihydroxyacetone/predicted adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,254,608 | 0 | T | C | 25.0% | 120.0 / 43.8 | 68 | intergenic (+46/+54) | dhaR/ycgV | predicted DNA‑binding transcriptional regulator, dihydroxyacetone/predicted adhesin |
| Reads supporting (aligned to +/- strand): ref base T (25/26); new base C (14/3); total (39/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAG > W3110S.gb/1254552‑1254674 | gcCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTaa > 1:4648407/1‑71 (MQ=255) gTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAAtt < 1:4035846/71‑1 (MQ=255) aTGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAAtttt > 1:1707536/1‑71 (MQ=255) tGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAAtttt > 1:2214039/1‑70 (MQ=255) tGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAAtttt > 1:4967671/1‑70 (MQ=255) tGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCAGGCGCACGCTAACAAtt < 1:4193761/68‑1 (MQ=255) gAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGc < 1:711493/59‑1 (MQ=255) gAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGc < 1:4445738/59‑1 (MQ=255) gAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGc < 1:54845/59‑1 (MQ=255) aaaGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGc < 1:211586/58‑1 (MQ=255) aaaGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAg > 1:1013009/1‑71 (MQ=255) aaaGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAAt > 1:4365789/1‑65 (MQ=255) aaaGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAg > 1:3949512/1‑71 (MQ=255) aGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa > 1:3304062/1‑71 (MQ=255) cAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCg > 1:3078217/1‑71 (MQ=255) cAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCg > 1:1962440/1‑71 (MQ=255) aGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCGt > 1:369874/1‑71 (MQ=255) gAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGtt > 1:4354077/1‑71 (MQ=255) aaaCGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGttt < 1:1321905/71‑1 (MQ=255) aCGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACATTTTTCAGCATCGttt < 1:2499612/69‑1 (MQ=255) cGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAg < 1:3614026/71‑1 (MQ=255) ttCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACaa > 1:2548489/1‑49 (MQ=255) ttCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa < 1:1074950/58‑1 (MQ=255) ttCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa < 1:1132468/58‑1 (MQ=255) ttCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCt > 1:509366/1‑71 (MQ=255) ttCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCt > 1:3793157/1‑71 (MQ=255) tCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTaa > 1:612544/1‑65 (MQ=255) tCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTaa > 1:3906829/1‑65 (MQ=255) tCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCt < 1:4516939/70‑1 (MQ=255) tCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCAAGCTAACAATTTTCAGCATCGTTTaa > 1:3790400/1‑65 (MQ=255) tGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTaa < 1:4169870/63‑1 (MQ=255) tGATACATCAGAGTGATCTGTATTTCATTCAGGCGCACGCTAACAATTTTCAGCATCGTTTaa < 1:1363685/63‑1 (MQ=255) aTACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTg < 1:3230006/68‑1 (MQ=255) aTACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTg < 1:4245431/68‑1 (MQ=255) aTACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCt < 1:3563600/71‑1 (MQ=255) tCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATccc > 1:535011/1‑71 (MQ=255) agagTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAg > 1:247429/1‑44 (MQ=255) agagTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAg > 1:3552388/1‑55 (MQ=255) gagTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATcc < 1:3221555/67‑1 (MQ=255) gTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCg > 1:3114633/1‑67 (MQ=255) tGATCTGTATTTCATTCCGGAGCACGCTAACAATTTTCa > 1:1711717/1‑39 (MQ=255) aTCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGc > 1:1433139/1‑39 (MQ=255) aTCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa < 1:3204355/40‑1 (MQ=255) aTCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa < 1:564868/40‑1 (MQ=255) aTCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTa < 1:3526543/70‑1 (MQ=255) cTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGtt > 1:381428/1‑43 (MQ=255) cTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGtt > 1:318547/1‑43 (MQ=255) cTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTaa > 1:4278766/1‑69 (MQ=255) tGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATc < 1:4823642/39‑1 (MQ=255) tGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATc < 1:477840/39‑1 (MQ=255) tGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATc < 1:4687244/39‑1 (MQ=255) tGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATc > 1:1341501/1‑58 (MQ=255) tGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAg > 1:5130922/1‑71 (MQ=255) tATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTaaaa < 1:3709719/68‑1 (MQ=255) tATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTaaa < 1:2317020/67‑1 (MQ=255) tATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTaaa < 1:2099215/67‑1 (MQ=255) tATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCt > 1:4137761/1‑71 (MQ=255) tttCATTCCGGCGCACGCTAACAATTTTCAGCATCGttt > 1:2396896/1‑39 (MQ=255) tttCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTg > 1:1786912/1‑70 (MQ=255) tttCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTg > 1:260836/1‑70 (MQ=255) tttCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTg < 1:4121916/70‑1 (MQ=255) tttCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTg < 1:126061/70‑1 (MQ=255) tttCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTg > 1:3404637/1‑70 (MQ=255) tttCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGc > 1:1019131/1‑45 (MQ=255) tCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTGTCCCGCACTTAAAAGCTGAAg > 1:2122335/1‑71 (MQ=255) tCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAg > 1:3322637/1‑71 (MQ=255) tCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTaa > 1:4920989/1‑39 (MQ=255) tCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTaa > 1:1077312/1‑39 (MQ=255) | GCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAG > W3110S.gb/1254552‑1254674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |