| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,255,918 | C→T | 38.4% | G538S (GGC→AGC) | ycgV ← | predicted adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,255,918 | 0 | C | T | 38.4% | 76.5 / 95.0 | 86 | G538S (GGC→AGC) | ycgV | predicted adhesin |
| Reads supporting (aligned to +/- strand): ref base C (30/23); new base T (19/14); total (49/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01 | |||||||||||
CGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGT > W3110S.gb/1255849‑1255987 | cGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGcc > 1:657292/1‑70 (MQ=255) aTTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCtaacaa > 1:5011570/1‑71 (MQ=255) cAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATc < 1:1743967/70‑1 (MQ=255) aGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAg > 1:1253171/1‑71 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAg < 1:2322129/67‑1 (MQ=255) actCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaa > 1:347494/1‑56 (MQ=255) actCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaa > 1:4420687/1‑56 (MQ=255) cccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCa > 1:866519/1‑61 (MQ=255) ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCataa > 1:5053784/1‑70 (MQ=255) ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCataa > 1:714281/1‑70 (MQ=255) ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCataa > 1:377506/1‑70 (MQ=255) ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCataa > 1:1115053/1‑70 (MQ=255) gCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCataata > 1:2215410/1‑70 (MQ=255) gCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCataata > 1:2169252/1‑70 (MQ=255) gCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCAtaataa < 1:1919902/71‑1 (MQ=255) tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATaaa < 1:4406820/70‑1 (MQ=255) atatTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAgg < 1:4166630/70‑1 (MQ=255) atatTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAgg < 1:4146262/70‑1 (MQ=255) atatTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAgg < 1:4115684/70‑1 (MQ=255) atatTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGt < 1:4914422/71‑1 (MQ=255) atTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTAc > 1:208723/1‑71 (MQ=255) atTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTAc > 1:4689269/1‑71 (MQ=255) ttCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCataata < 1:3277546/63‑1 (MQ=255) cAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAacaaca > 1:4745341/1‑46 (MQ=255) cAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGt > 1:32806/1‑71 (MQ=255) cAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGt > 1:3169932/1‑71 (MQ=255) aaTAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGt < 1:1556937/70‑1 (MQ=255) aTAAATCCCCTTTATTATTAACGCCATTACCCTCGCtaac < 1:33386/40‑1 (MQ=255) aTAAATCCCCTTTATTATTAACGCCATTACCCTCGCtaac < 1:1130382/40‑1 (MQ=255) aTAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGt < 1:89285/69‑1 (MQ=255) aTAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAt < 1:1321693/45‑1 (MQ=255) aTAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCAtaataa < 1:4778588/60‑1 (MQ=255) aTAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCAtaataa < 1:1487483/60‑1 (MQ=255) tAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAgaa > 1:1961585/1‑47 (MQ=255) tAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCa > 1:1787084/1‑49 (MQ=255) tAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCa > 1:733414/1‑49 (MQ=255) tAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAc > 1:2484420/1‑71 (MQ=255) tAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAc > 1:2894916/1‑71 (MQ=255) aaaTCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc > 1:1909934/1‑71 (MQ=255) aaaTCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAcc > 1:2992918/1‑71 (MQ=255) tCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTa > 1:3407248/1‑66 (MQ=255) tCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTa > 1:1141424/1‑66 (MQ=255) tCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTa > 1:2556044/1‑66 (MQ=255) ccccTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGc > 1:3120673/1‑47 (MQ=255) cccTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCa < 1:4108819/71‑1 (MQ=255) ccTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCataat < 1:3796095/50‑1 (MQ=255) ccTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCataat < 1:2469055/50‑1 (MQ=255) ccTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCataat < 1:2234300/50‑1 (MQ=255) cTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAgg < 1:1074957/71‑1 (MQ=255) ttattattaACGCCATTACCCTCGCTAACAACATCAGc < 1:475457/38‑1 (MQ=255) ttattattaACGCCATTACCCTCGCTAACAACATCAGc < 1:1108980/38‑1 (MQ=255) ttattattaACGCCATTACCCTCGCCAACAACATCAg < 1:821731/37‑1 (MQ=255) ttattattaACGCCATTACCCTCGCCAACAACATCAg < 1:620954/37‑1 (MQ=255) ttattattaACGCCATTACCCTCGCCAACAACATCAg < 1:2672858/37‑1 (MQ=255) tattattaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtc > 1:1935632/1‑71 (MQ=255) tattattaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtc > 1:1209654/1‑71 (MQ=255) attattaACGCCATTACCCTCGCCAACAACATCAGCa > 1:3744912/1‑37 (MQ=255) ttaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATaaa < 1:1213257/44‑1 (MQ=255) ttaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTc > 1:2451414/1‑60 (MQ=255) ttaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTc > 1:301035/1‑60 (MQ=255) ttaACGCCATTACCCTCGCTAACAACATCAGAACGCATAATaaa < 1:3725688/44‑1 (MQ=38) aaCGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAgg > 1:325205/1‑44 (MQ=255) aaCGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAgg > 1:2139013/1‑44 (MQ=255) aaCGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAgg > 1:3665220/1‑44 (MQ=255) aaCGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAgg > 1:3507701/1‑44 (MQ=255) aCGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGtt < 1:708290/71‑1 (MQ=255) aCGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGtt < 1:3314163/71‑1 (MQ=255) cGCCATTACCCTCGCCCACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGtt > 1:1393173/1‑70 (MQ=255) cGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGtt > 1:2097646/1‑70 (MQ=255) ccATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTaa > 1:3520926/1‑71 (MQ=255) ccATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTaa > 1:2214534/1‑71 (MQ=255) ccATTACCCTCGCCAACAACATCAGCACGCATAATAAAGATACTGTTACCGCTCAGGTTCTCTACGTTTaa > 1:3190596/1‑71 (MQ=255) aTTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGt < 1:650763/44‑1 (MQ=255) aTTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg < 1:3152047/71‑1 (MQ=255) aTTACCCTCGCTAACAAAATCAGCACGCATAATAAAGGTACTGTTAc > 1:3472330/1‑47 (MQ=255) aTTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg < 1:2835953/71‑1 (MQ=255) aTTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg < 1:2099628/71‑1 (MQ=255) tACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg > 1:1056069/1‑71 (MQ=255) aCCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg > 1:4871634/1‑70 (MQ=255) aCCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg > 1:2585583/1‑70 (MQ=255) aCCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgtg > 1:3827283/1‑70 (MQ=255) aCCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTgg > 1:4521116/1‑71 (MQ=255) aCCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTgg > 1:3444933/1‑71 (MQ=255) cTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCaa < 1:4687336/71‑1 (MQ=255) cTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCaa < 1:569845/71‑1 (MQ=255) cTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCaa < 1:2498455/71‑1 (MQ=255) tCGCTAACAACATCAGCACGCATAATAAAGGTACTGt < 1:2591898/37‑1 (MQ=255) tCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGtt > 1:4244871/1‑50 (MQ=255) tCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGtt > 1:3417559/1‑50 (MQ=255) tCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTaa < 1:1169226/62‑1 (MQ=255) gCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTa > 1:3244727/1‑53 (MQ=255) gCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACg > 1:2688150/1‑55 (MQ=255) ccAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAAtgt < 1:1722542/71‑1 (MQ=255) | CGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGT > W3110S.gb/1255849‑1255987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |