| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,274,854 | G→A | 29.1% | T108M (ACG→ATG) | ychN ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,274,854 | 0 | G | A | 29.1% | 85.4 / 39.9 | 55 | T108M (ACG→ATG) | ychN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (20/19); new base A (9/7); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACT > W3110S.gb/1274786‑1274915 | ttCCCGCATAGTGGGGCGTCAGCCGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTc > 1:4120293/1‑71 (MQ=255)ttCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTc > 1:3950916/1‑71 (MQ=255)ttCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTc > 1:3056311/1‑71 (MQ=255) cccGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCa > 1:4258232/1‑71 (MQ=255) cccGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATcc < 1:4398177/70‑1 (MQ=255) tAGTGGGGCGTCAGACTCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTcc > 1:3513570/1‑64 (MQ=255) tAGTGGGGCGTCAGACTCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCa > 1:1294253/1‑65 (MQ=255) gggCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGtt > 1:4767639/1‑71 (MQ=255) aGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTgc > 1:1206597/1‑57 (MQ=255) cccTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTcc < 1:4461428/45‑1 (MQ=255) cccTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTcc < 1:2191821/45‑1 (MQ=255) cAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGAttt > 1:435499/1‑71 (MQ=255) aaaCATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTc < 1:2593040/71‑1 (MQ=255) aaaCATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTc < 1:2686817/71‑1 (MQ=255) aaaCATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTc < 1:1941135/71‑1 (MQ=255) aaaCATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTc < 1:3294131/71‑1 (MQ=255) aaCATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTc < 1:2782814/70‑1 (MQ=255) ttAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTgc < 1:434722/40‑1 (MQ=255) ttAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCca < 1:4899589/50‑1 (MQ=255) tAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAccc > 1:3912636/1‑70 (MQ=255) aaaaTGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCATAaga > 1:70815/1‑53 (MQ=255) aaaaTGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAaga > 1:4932695/1‑53 (MQ=255) aaaaTGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAcccc > 1:3298882/1‑70 (MQ=255) aaaaTGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAcccaa > 1:4061497/1‑69 (MQ=255) aaaaTGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGa > 1:3609122/1‑60 (MQ=255) aaaaTGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGa > 1:1169498/1‑60 (MQ=255) aaaaTGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGa > 1:1492793/1‑60 (MQ=255) aaaaTGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCa > 1:322935/1‑71 (MQ=255) aaaTGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCat > 1:4070407/1‑71 (MQ=255) aaaTGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAACCCa > 1:730911/1‑70 (MQ=255) aaaTGTGAGAACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCat > 1:3907756/1‑71 (MQ=255) cACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaat < 1:1773167/66‑1 (MQ=255) cACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaat < 1:2122691/66‑1 (MQ=255) cACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaat < 1:2399388/66‑1 (MQ=255) aCTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAgg < 1:1153263/71‑1 (MQ=255) cTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGAt < 1:3431865/49‑1 (MQ=255) cTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaatca > 1:2768454/1‑66 (MQ=255) cTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaatca > 1:4392307/1‑66 (MQ=255) tttATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGAt < 1:3617477/48‑1 (MQ=255) tATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAagag < 1:2439749/39‑1 (MQ=255) tATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAagag < 1:3341269/39‑1 (MQ=255) tATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTa > 1:1870373/1‑41 (MQ=255) tATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAccc > 1:4227737/1‑54 (MQ=255) tATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAccc > 1:4235861/1‑54 (MQ=255) tCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAg > 1:340008/1‑68 (MQ=255) cGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAgtg < 1:4089749/69‑1 (MQ=255) gCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACt < 1:4564310/71‑1 (MQ=255) gACAGCGTCCATTGCGCCAGTTCCACAAGAGTAcc < 1:4797598/35‑1 (MQ=255) gACAGCGTCCATTGCGCCAGTTCCACAAGAGTAcc < 1:3098801/35‑1 (MQ=255) aCAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACt < 1:1798726/67‑1 (MQ=255) aCAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACt < 1:1804564/67‑1 (MQ=255) aCAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACt < 1:5124880/67‑1 (MQ=255) aCAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACt < 1:556156/67‑1 (MQ=255) cAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGa > 1:809289/1‑35 (MQ=255) cAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGa > 1:815237/1‑35 (MQ=255) | TTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACT > W3110S.gb/1274786‑1274915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |