| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,287,091 | C→T | 31.6% | Q126* (CAA→TAA) | narJ → | molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,287,091 | 0 | C | T | 31.6% | 50.3 / 30.1 | 38 | Q126* (CAA→TAA) | narJ | molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1 |
| Reads supporting (aligned to +/- strand): ref base C (12/14); new base T (9/3); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTG > W3110S.gb/1287024‑1287156 | gCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGTaa < 1:1423997/70‑1 (MQ=255)gCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCaa < 1:2758806/70‑1 (MQ=255) ttAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCaa < 1:2604898/66‑1 (MQ=255) tAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCg > 1:1030850/1‑69 (MQ=255) aaaCAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAg > 1:1735985/1‑71 (MQ=255) ccGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGTAAAGCGAAGCCGTg > 1:737404/1‑70 (MQ=255) cgcgAATTGCCGGACCATCTGCCGCTGTAACTGGAGTACCTGGCGCAGCTGCCGTAAAGCGAAGCCGTgg > 1:2075302/1‑70 (MQ=255) ttGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGTAAAGCGAAGCCGTGGAAGGttt > 1:4632783/1‑71 (MQ=255) gCCGGACCATCTGCCGCTGTATCTGGAGTATCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTg > 1:1064095/1‑70 (MQ=255) cGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGaaa < 1:4236711/71‑1 (MQ=255) gACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGa < 1:2716320/71‑1 (MQ=255) aCCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGTAAAGCGAAGCCGTGGAAGGTTTGAAAGat < 1:2734829/71‑1 (MQ=255) cGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCg > 1:2169593/1‑45 (MQ=255) gTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGa > 1:3174802/1‑57 (MQ=255) tATCTGGAGTACCTGGCGCAGCTGCCGTAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTc < 1:1274052/70‑1 (MQ=255) aTCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTg > 1:1684623/1‑71 (MQ=255) tGGATTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGcc > 1:702443/1‑61 (MQ=255) ggAGTACCTGGCGCAGCTGCCGTAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGAtt > 1:1054357/1‑64 (MQ=255) gAGTACCTGGCGCAGCTGCCGCAAAGTGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTgg < 1:4173479/67‑1 (MQ=255) gAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTgg < 1:3680028/67‑1 (MQ=255) gAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTgg < 1:3987280/67‑1 (MQ=255) gAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTgg < 1:3993282/67‑1 (MQ=255) gAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGc < 1:857712/68‑1 (MQ=255) gTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGc > 1:2363425/1‑71 (MQ=255) cTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTgg < 1:2166163/61‑1 (MQ=255) tGGCGCAGCTGCCGTAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAgc > 1:1952014/1‑71 (MQ=255) ggCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGatat > 1:4950852/1‑45 (MQ=255) gcAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAgcgcgc < 1:499200/71‑1 (MQ=255) tGCCGTAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTg > 1:2186482/1‑71 (MQ=255) tGCCGTAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTg > 1:4861081/1‑71 (MQ=255) gCCGTAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTc > 1:832481/1‑47 (MQ=255) gCCGTAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTc > 1:1724560/1‑47 (MQ=255) gCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCAtt > 1:2475510/1‑54 (MQ=255) gCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGa > 1:4724691/1‑59 (MQ=255) gCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGa > 1:2616757/1‑59 (MQ=255) cGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCAt < 1:61477/51‑1 (MQ=255) cGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCAt < 1:537460/51‑1 (MQ=255) cGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCAt < 1:4796252/51‑1 (MQ=255) | GCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGCAGCTGCCGCAAAGCGAAGCCGTGGAAGGTTTGAAAGATATCGCGCCGATTCTGGCATTGCTGAGCGCGCGTCTG > W3110S.gb/1287024‑1287156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |