| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,352,881 | T→C | 34.8% | intergenic (‑128/+240) | fabI ← / ← ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,352,881 | 0 | T | C | 34.8% | 18.4 / 19.3 | 23 | intergenic (‑128/+240) | fabI/ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (8/7); new base C (4/4); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
GTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGG > W3110S.gb/1352814‑1352945 | gTAAACAGTACGAACAGATAAACTGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCg < 1:4658997/71‑1 (MQ=255) aaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGAc > 1:230141/1‑70 (MQ=255) aaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGAcc > 1:4227480/1‑71 (MQ=255) cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCgg < 1:46630/71‑1 (MQ=255) agaaCGAACAGATAAACGGTTATTATAATCAAACTCGCTGTGAGTAGCTATAGTTGCCAGGTCCGa > 1:2764128/4‑66 (MQ=255) aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCg < 1:3954399/66‑1 (MQ=255) aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAg > 1:1091770/1‑69 (MQ=255) cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCGGAGCAg > 1:4815206/1‑71 (MQ=255) gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCa < 1:2295641/69‑1 (MQ=255) tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCGGAGCAGGCTGc > 1:2614607/1‑68 (MQ=255) aaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCgg < 1:550167/68‑1 (MQ=255) cGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCa > 1:381436/1‑68 (MQ=255) ggTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCg < 1:4659917/48‑1 (MQ=255) ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCg > 1:1873216/1‑47 (MQ=255) ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCg > 1:2612090/1‑47 (MQ=255) taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGc > 1:708069/1‑48 (MQ=255) taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGc > 1:3724015/1‑48 (MQ=255) taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCtt < 1:4723143/71‑1 (MQ=255) taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCtt < 1:4895942/71‑1 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCt < 1:4239563/71‑1 (MQ=255) tGGCTGTGAGTAGCTATAGTTGCCAGGCCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTccc > 1:4848596/1‑67 (MQ=255) tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTa < 1:4885069/70‑1 (MQ=255) ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg < 1:5069758/70‑1 (MQ=255) | GTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGG > W3110S.gb/1352814‑1352945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |