| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,380 | 2 bp→AA | 100% | pseudogene (513‑514/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,380 | 0 | G | A | 100.0% | 172.9 / NA | 58 | pseudogene (513/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/31); total (27/31) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | W3110S.gb | 3,824,381 | 0 | G | A | 100.0% | 169.3 / NA | 58 | pseudogene (514/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/31); total (27/31) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGAC > W3110S.gb/3824315‑3824442 || cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg > 1:2994338/1‑71 (MQ=255) aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa > 1:2824550/1‑71 (MQ=255) aaccaaccCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATc > 1:562265/1‑71 (MQ=255) aCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg < 1:2761375/67‑1 (MQ=255) aCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCt > 1:3451634/1‑71 (MQ=255) ccAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCt < 1:578141/70‑1 (MQ=255) ccAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTg < 1:4912872/71‑1 (MQ=255) ccAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTg < 1:2390956/71‑1 (MQ=255) ccAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTg < 1:5107184/71‑1 (MQ=255) ccAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTg < 1:4152608/71‑1 (MQ=255) acccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa < 1:1333910/69‑1 (MQ=255) ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACTGTGAAGCAATTTGCGATCTGGAATa > 1:4762662/1‑71 (MQ=255) ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa > 1:173370/1‑71 (MQ=255) cGATGAAAGGGATGGTAGCCGCAGTTTCCGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATAc < 1:4271093/71‑1 (MQ=255) gATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACg > 1:90940/1‑71 (MQ=255) gAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa > 1:4400866/1‑66 (MQ=255) gAAAGGGATGGTAGACGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa > 1:104257/1‑66 (MQ=255) aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg < 1:241735/70‑1 (MQ=255) aaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg > 1:2291937/1‑69 (MQ=255) aaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa < 1:634891/71‑1 (MQ=255) aaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa < 1:2220482/71‑1 (MQ=255) gggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGa < 1:2845717/71‑1 (MQ=255) ggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATAc < 1:3977246/62‑1 (MQ=255) gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt > 1:4703860/1‑60 (MQ=255) gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg < 1:4977454/61‑1 (MQ=255) gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg < 1:3081076/61‑1 (MQ=255) gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGActcag < 1:509473/70‑3 (MQ=255) aGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg > 1:2543418/1‑71 (MQ=255) gCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa > 1:3760291/1‑44 (MQ=21) gCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa > 1:3796225/1‑44 (MQ=21) gCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa > 1:4224767/1‑44 (MQ=21) ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt < 1:3987026/49‑1 (MQ=25) ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt < 1:2549385/49‑1 (MQ=25) tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCg > 1:3131981/1‑70 (MQ=255) cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa > 1:4881398/1‑70 (MQ=255) cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa > 1:4875778/1‑70 (MQ=255) cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa > 1:4284490/1‑70 (MQ=255) cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa < 1:2047520/70‑1 (MQ=255) cGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATg > 1:3211571/1‑70 (MQ=255) cGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATg > 1:3517274/1‑70 (MQ=255) ggtaTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGaa > 1:581693/4‑71 (MQ=255) ggtaTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGaa < 1:1137145/68‑1 (MQ=255) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACg < 1:733189/36‑1 (MQ=16) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACg < 1:1745358/36‑1 (MQ=16) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc < 1:4725043/49‑1 (MQ=25) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc < 1:2957902/49‑1 (MQ=25) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCaga < 1:3367154/55‑1 (MQ=255) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCaga < 1:2911979/55‑1 (MQ=255) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg > 1:3687203/1‑69 (MQ=255) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt < 1:3356189/70‑1 (MQ=255) tgtaAACGGTGAAGCAATTTGCGATCTGGAATACg < 1:5094879/35‑1 (MQ=16) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt > 1:4231358/4‑71 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt > 1:2492810/4‑71 (MQ=255) taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt < 1:4883736/68‑1 (MQ=255) aaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgat < 1:2442030/71‑1 (MQ=255) aCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgat > 1:3949146/1‑70 (MQ=255) gtgAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGAc > 1:3633823/3‑70 (MQ=255) gAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg < 1:225267/40‑1 (MQ=37) || CTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGAC > W3110S.gb/3824315‑3824442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |