Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,281,798	Δ1 bp	intergenic (+318/‑33)	agaA → / → agaS	predicted truncated N‑acetylgalactosamine‑6‑phosphate deacetylase/tagatose‑6‑phosphate ketose/aldose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,281,796	0	C	.	100.0%	304.0 / NA	79	intergenic (+316/‑35)	agaA/agaS	predicted truncated N‑acetylgalactosamine‑6‑phosphate deacetylase/tagatose‑6‑phosphate ketose/aldose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (0/79);  total (0/79)

CACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCAT  >  W3110S.gb/3281754‑3281812                                           |                 cACCAAGTGAGATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:8180/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:68516/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:70212/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:68453/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:68095/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:65726/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:63308/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:62076/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:6142/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:59471/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:57621/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:50386/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:49918/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:47858/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:47594/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:47542/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:11059/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:47095/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:44002/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:81480/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:9247/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:87204/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:87084/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:85721/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:84907/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:84166/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:84065/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:83977/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:47157/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:81197/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:78429/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:77631/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:75695/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:73382/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:72734/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:7079/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:16575/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:23480/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:41493/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:20873/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:20536/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:1987/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:19856/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:17769/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:1704/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:16688/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:20930/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:16140/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:15630/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:13913/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:13797/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:13658/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:12679/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:12672/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:12497/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:33431/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:39757/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:38874/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:38773/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:37224/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:36576/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:35352/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:34537/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:23987/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:31821/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:29588/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:27310/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:25712/58‑1 (MQ=255) cACCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:24793/58‑1 (MQ=255) cACCAAGTGAAACGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:13420/58‑1 (MQ=255)  aCCAAGTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:8681/57‑1 (MQ=255)       gTGAAATGAAACGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:57544/52‑1 (MQ=255)         gaaatgaaaCGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:58505/50‑1 (MQ=255)         gaaatgaaaCGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:21883/50‑1 (MQ=255)          aaatgaaaCGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:53882/49‑1 (MQ=38)           aatgaaaCGAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:74395/48‑1 (MQ=38)                   gAAAGGCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:83526/40‑1 (MQ=37)                    aaaGCCAAGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:47352/39‑1 (MQ=25)                       ggCATGCGAAAGCGACAACG‑CCGACGTCAAGTtcat  <  1:16882/36‑1 (MQ=21)                                           |                 CACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCAT  >  W3110S.gb/3281754‑3281812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑