| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 871,017 | +A | coding (1185/1539 nt) | yliB → | predicted peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 871,014 | 1 | . | A | 96.2% | 40.1 / ‑5.9 | 26 | G394G (GGT→GGA) | yliB | predicted peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base A (0/25); minor base G (0/1); total (0/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGT‑AAAGGGCAAAAAGAGAGCGGC > W3110S.gb/870975‑871035 | tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTGAAAGGGCAAAAAGAGAgcggc < 1:39156/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:52190/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:82106/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:75430/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:73921/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:73397/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:72344/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:70811/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:68302/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:66259/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:63479/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:5942/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:57988/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:10708/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:51132/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:48091/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:36097/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:36011/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:32732/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:28384/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:26993/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:2396/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:20080/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:19060/62‑1 (MQ=255)tGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:12781/62‑1 (MQ=255) gCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGTAAAAGGGCAAAAAGAGAgcggc < 1:57865/61‑1 (MQ=255) | TGCGATGGATGCCGGACAGCGGGCGGCAGAAGTTGAAGGT‑AAAGGGCAAAAAGAGAGCGGC > W3110S.gb/870975‑871035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |