Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,343,430	+C	coding (1775/2271 nt)	adiA ←	biodegradative arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,343,426	1	.	C	100.0%	13.5 / NA	10	G593G (GGA→GGG)	adiA	biodegradative arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (10/0);  total (10/0)

CAAAGGGTGTTAACCAGAGTT‑CCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAC  >  W3110S.gb/4343406‑4343465                      |                                        cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGccc                 >  1:56662/1‑46 (MQ=38) cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGaa   >  1:30746/1‑60 (MQ=255) cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc  >  1:47348/1‑61 (MQ=255) cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc  >  1:54051/1‑61 (MQ=255) cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc  >  1:73404/1‑61 (MQ=255) cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc  >  1:74590/1‑61 (MQ=255) cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc  >  1:80801/1‑61 (MQ=255) cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc  >  1:81913/1‑61 (MQ=255) cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc  >  1:83315/1‑61 (MQ=255) cAAAGGGTGTTAACCAGAGTTCCCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAc  >  1:8941/1‑61 (MQ=255)                      |                                        CAAAGGGTGTTAACCAGAGTT‑CCCCATTTCCCACGGGTTACGCCCATAGAGAACAGGAAC  >  W3110S.gb/4343406‑4343465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑