Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,263,2030CT44.1% 1.7 / 14.4 34P514P (CCG→CCA) fruAfused fructose‑specific PTS enzyme IIB'BC components
Reads supporting (aligned to +/- strand):  ref base C (19/0);  new base T (15/0);  total (34/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AACCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCACC  >  W3110S.gb/2263148‑2263223
                                                       |                    
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:16762/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:79886/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:71738/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:69597/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:64950/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:54096/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:5275/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:51378/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:50379/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:47853/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:44050/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:34288/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:33847/1‑62 (MQ=255)
aaCCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:32999/1‑62 (MQ=255)
aaCCCAATACAGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGTGGTGcc                >  1:63605/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGGAcc  >  1:78194/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGGAcc  >  1:78093/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAcc  >  1:4399/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAcc  >  1:84996/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAcc  >  1:78931/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAcc  >  1:76923/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAcc  >  1:75589/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAcc  >  1:73374/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAcc  >  1:20229/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAcc  >  1:49656/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAcc  >  1:48936/1‑62 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAc   >  1:20357/1‑61 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAc   >  1:20490/1‑61 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAc   >  1:54625/1‑61 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAc   >  1:2720/1‑61 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAc   >  1:16037/1‑61 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAc   >  1:83101/1‑61 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAc   >  1:84504/1‑61 (MQ=255)
              gTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCAc   >  1:85713/1‑61 (MQ=255)
                                                       |                    
AACCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAACAAACAGACCACCGTGCGGTGCCATCAGTTTCGCACC  >  W3110S.gb/2263148‑2263223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑