| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,448,169 | 0 | G | A | 61.9% | 11.5 / 14.2 | 42 | E320E (GAG→GAA) | trmC | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Reads supporting (aligned to +/- strand): ref base G (16/0); new base A (26/0); total (42/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGT > W3110S.gb/2448114‑2448215 | gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:53438/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:34559/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:38069/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:40990/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:12334/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:43893/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:49460/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:51268/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:52336/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:41405/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:58588/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:6445/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:67185/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:70450/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:75940/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:80021/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:80227/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:20586/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:12524/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:13746/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:30772/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:22233/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:23056/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:23488/1‑62 (MQ=255)gTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:25231/1‑62 (MQ=255)gTGCTTCCGGCAATCGACAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAAGCGCTa > 1:68083/1‑62 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATg > 1:22008/1‑35 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCgg > 1:46465/1‑55 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:17693/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:35836/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:13679/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:7793/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:30021/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:72094/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:14989/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:67532/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:66931/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:18146/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:6411/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:2778/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:24889/1‑56 (MQ=255) acacGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGt > 1:44220/1‑56 (MQ=255) | GTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGT > W3110S.gb/2448114‑2448215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |