| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,679,431 | 0 | G | T | 72.6% | 62.7 / 25.2 | 62 | R657R (CGC→CGA) | yphG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/17); new base T (45/0); total (45/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.35e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACACAGACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGTT > W3110S.gb/2679379‑2679475 | tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:66404/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:50515/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:51813/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:53606/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:54718/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:56136/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:57261/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:61619/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:61812/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:62669/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:63298/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:64455/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:64776/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:66042/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:15685/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:67836/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:67868/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:68918/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:69563/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:73150/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:76434/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:76447/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:81134/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:81479/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:82566/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:85670/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:87113/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:87896/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:1535/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:47852/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:18893/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:20975/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:25630/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:457/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:26835/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:28110/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:44833/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc > 1:31044/1‑62 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg > 1:34570/1‑61 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg > 1:9618/1‑61 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg > 1:1870/1‑61 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg > 1:34413/1‑61 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg > 1:35086/1‑61 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg > 1:47740/1‑61 (MQ=255)tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg > 1:55353/1‑61 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:4501/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:50049/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:50646/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:47788/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:20157/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:46054/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:79596/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:77803/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:35510/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:61773/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:29123/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:40307/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:33040/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:33387/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:37889/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:50326/49‑1 (MQ=255) gACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGtt < 1:34893/49‑1 (MQ=255) | TCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACACAGACCGCGCCACTGCGCGAGAGCTGATTCGCTACGACCATCGAACCAGTT > W3110S.gb/2679379‑2679475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |