Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb110,4270GT28.7% 130.3 / 47.9 115D717Y (GAT→TAT) secApreprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon
Reads supporting (aligned to +/- strand):  ref base G (47/35);  new base T (0/33);  total (47/68)
Fisher's exact test for biased strand distribution p-value = 4.45e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 115 total aligned reads displayed.

ATATTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAC  >  W3110S.gb/110383‑110460
                                            |                                 
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAAt                         >  1:45373/1‑55 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGa                   >  1:10106/1‑61 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGa                   >  1:85134/1‑61 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:38209/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:80111/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:79511/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:79468/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:33302/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:73795/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:34012/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:70970/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:69055/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:68825/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:67438/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:66703/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:30966/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:60665/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:42911/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:43035/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:6019/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:57634/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:54489/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:49744/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:21468/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:10425/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:14864/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:16881/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:17028/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:17200/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:21280/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:80911/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:82658/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:2263/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:23284/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:28425/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:82612/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:81829/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:27857/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAACTGCCGAg                  >  1:12439/1‑62 (MQ=255)
atatTCCGGGGCTGCAGGAACGTCTGAAGAAAGATTTCGACCTCGATTTGCCAATTGCCGa                   >  1:26165/1‑61 (MQ=255)
atatTCCGGGGCTGAAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAg                  >  1:5649/1‑62 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:45837/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:54068/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:87393/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:85814/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:8531/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:5903/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:79364/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:71730/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:77389/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:60994/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:76359/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:63991/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:83222/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:69975/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:47369/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:32465/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:31943/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:30730/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:35399/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:35596/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:36785/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:28677/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:37911/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:21732/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:3988/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:44595/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:17507/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:22260/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:23921/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:26999/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:80952/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:14291/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:29605/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:12558/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:29932/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:86747/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:30443/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:11914/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:49674/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:8895/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:33933/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:46393/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:46304/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:45198/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:43282/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:41903/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:61110/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:61700/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:63529/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:64076/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:65866/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:66310/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:36861/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:36584/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:3436/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:71862/52‑1 (MQ=255)
                          aagaaCGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:77247/52‑1 (MQ=255)
                           agaaCGATTTCGACCTCTATTTGCCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:82442/51‑1 (MQ=255)
                           agaaCGATTTCGACCTCGATTT‑CCAATTGCCGAGTGGCTGGATAAAGAAc  <  1:36892/50‑1 (MQ=38)
                                            |                                 
ATATTCCGGGGCTGCAGGAACGTCTGAAGAACGATTTCGACCTCGATTTGCCAATTGCCGAGTGGCTGGATAAAGAAC  >  W3110S.gb/110383‑110460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑