| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 739,718 | 0 | T | C | 51.0% | ‑0.9 / 25.0 | 49 | V99A (GTT→GCT) | ybgA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/24); new base C (25/0); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTTTGCTTTCTCACCCCGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATA > W3110S.gb/739663‑739741 | gttTTGCTTTCTCACCCTGCCAAGCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:10003/1‑61 (MQ=19)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:66968/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:73330/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:44953/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:44076/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:42433/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:41939/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:57392/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:7562/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:38747/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:38007/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:37739/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:36259/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:78056/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:23101/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:61633/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:16165/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:62210/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:12964/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg > 1:87877/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgc > 1:81849/1‑60 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAg > 1:61305/1‑60 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGAACGCTCAgg > 1:35208/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCAAACAAACGTTTTGATGCACGCTCAgg > 1:18319/1‑61 (MQ=255)gttTTGCTTTCTCACCCTGCCAACCGGCG‑TATCATACTAACGTTTTGATGCACGCTCAggga > 1:36065/1‑61 (MQ=255) tgCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:9742/61‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:74479/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:77362/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:82890/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:87321/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:7255/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:70123/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:48334/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:69945/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:69739/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:69474/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:68709/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:6545/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:6469/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:53535/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:40875/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:40164/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:27149/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:26489/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:22580/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:13366/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:11916/62‑1 (MQ=255) cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:10661/62‑1 (MQ=255) gCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata < 1:1933/61‑1 (MQ=255) | GTTTTGCTTTCTCACCCCGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATA > W3110S.gb/739663‑739741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |