Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	613,940	C→T	S187S (AGC→AGT)	entF →	enterobactin synthase multienzyme complex component, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	613,940	0	C	T	100.0%	163.8 / NA	87	S187S (AGC→AGT)	entF	enterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (87/0);  total (87/0)

AAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAG  >  W3110S.gb/613915‑613974                          |                                   aaGAGTATCAGCAGTACCGCGAAAGTGAATCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:59835/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCgg              >  1:60451/1‑48 (MQ=37) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTggg     >  1:39225/1‑57 (MQ=38) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTg       >  1:19790/1‑55 (MQ=38) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGTGcgg  >  1:53648/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:60045/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:64818/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:6439/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:64070/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:63079/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:62164/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:61928/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:60905/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:67082/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:57498/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:53720/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:54148/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:56910/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:59368/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:59253/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:58183/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:57863/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:57503/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:73843/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:9528/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:88158/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:87185/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:86043/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:85889/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:84443/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:81265/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:76334/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:74887/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:73929/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:65224/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:73387/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:72819/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:7276/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:72141/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:70359/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:69454/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:69101/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:69020/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:68549/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:50810/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:18174/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:27382/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:25213/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:24439/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:23776/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:23048/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:22990/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:21787/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:20794/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:20455/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:20242/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:2881/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:17451/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:16635/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:15755/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:14638/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:14544/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:12834/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:1262/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:12084/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:1173/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:37203/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:50471/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:47752/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:47642/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:46460/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:4276/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:40911/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:39976/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:39701/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:39362/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:3795/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:11631/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:37184/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:37170/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:36622/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:35730/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:34782/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:32928/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:31836/1‑58 (MQ=255) aaGAGTATCAGCAGTACCGCGAAAGTGAAGCCTGGCAGCGTGATGCGGCATTCTGGGcgg  >  1:30183/1‑58 (MQ=255) aaGAGTATCAGCAGTAACGCGAAAGTGAAGCCTGGCAGCGTGATGAGGCATTCTg       >  1:6475/1‑55 (MQ=37)                          |                                   AAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAG  >  W3110S.gb/613915‑613974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑