Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	669,940	G→T	R130R (CGC→CGA)	cobC ←	predicted alpha‑ribazole‑5'‑P phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	669,940	0	G	T	100.0%	50.1 / NA	29	R130R (CGC→CGA)	cobC	predicted alpha‑ribazole‑5'‑P phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/0);  total (29/0)

GATGGCTGACGACTAAAATATTCTGATAGTGCTGAAATTCACTAAGCCTTGCGATAAAGCGT  >  W3110S.gb/669882‑669943                                                           |    gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:37332/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:87108/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:8679/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:78464/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:7533/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:74235/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:72677/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:71092/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:68016/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:66150/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:61609/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:59505/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:50035/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:48234/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:47690/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:11213/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:29542/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:29412/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:28468/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:26602/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:25873/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:25201/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:22118/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:21472/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:17058/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:15175/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:14627/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTGATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:14488/1‑62 (MQ=255) gATGGCTGACGACTAAAATATTCTAATAGTGCTGATATTCACTAAGCCTTGCGATAAATCGt  >  1:20391/1‑62 (MQ=255)                                                           |    GATGGCTGACGACTAAAATATTCTGATAGTGCTGAAATTCACTAAGCCTTGCGATAAAGCGT  >  W3110S.gb/669882‑669943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑