Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	729,089	A→G	P22P (CCT→CCC)	kdpA ←	potassium translocating ATPase, subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	729,089	0	A	G	100.0%	69.9 / NA	39	P22P (CCT→CCC)	kdpA	potassium translocating ATPase, subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/0);  total (39/0)

CCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGA  >  W3110S.gb/729077‑729138             |                                                  cccGCTACCTAAGGGATGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:71055/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATTCCAGTAAAAACGTGGCAATCAGTAAGa  >  1:75975/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:68023/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:55309/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:57926/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:58081/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:59853/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:62482/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:62913/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:64220/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:64225/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:66976/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:51040/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:68921/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:69202/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:70012/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:76440/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:8299/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:8454/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:45962/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:15963/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:16034/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:16193/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:17015/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:21465/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:21912/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:22955/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:26671/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:34633/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:36459/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:38347/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:39107/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:39907/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:41032/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:43344/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:44797/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:10427/1‑62 (MQ=255) cccGCTACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGG‑AATCAGTAAGa  >  1:12567/1‑61 (MQ=255) cccGCCACCTAAGGGACGCGCCAGCACCATTAATACCAGTAAAAACGTGGCAATCAGTAAGa  >  1:73915/1‑62 (MQ=255)             |                                                  CCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGA  >  W3110S.gb/729077‑729138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑