Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	739,680	C→T	P86P (CCC→CCT)	ybgA →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	739,680	0	C	T	100.0%	41.7 / NA	25	P86P (CCC→CCT)	ybgA	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/0);  total (25/0)

GTTTTGCTTTCTCACCCCGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATA  >  W3110S.gb/739663‑739741                  |                                                              gttTTGCTTTCTCACCCTGCCAAGCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:10003/1‑61 (MQ=19) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:87877/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:66968/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:62210/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:61633/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:57392/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:73330/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:44953/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:44076/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:42433/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:41939/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:7562/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:38747/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:38007/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:36259/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:12964/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:16165/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:23101/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:78056/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgg                    >  1:37739/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAgc                    >  1:81849/1‑60 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGCACGCTCAg                     >  1:61305/1‑60 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCATACTAACGTTTTGATGAACGCTCAgg                    >  1:35208/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCGTGATCAAACAAACGTTTTGATGCACGCTCAgg                    >  1:18319/1‑61 (MQ=255) gttTTGCTTTCTCACCCTGCCAACCGGCG‑TATCATACTAACGTTTTGATGCACGCTCAggga                  >  1:36065/1‑61 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:77362/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:74479/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:82890/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:87321/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:7255/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:70123/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:48334/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:69945/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:69739/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:69474/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:68709/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:6545/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:6469/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:53535/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:40875/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:40164/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:27149/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:26489/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:22580/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:13366/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:11916/62‑1 (MQ=255)                  cGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCata  <  1:10661/62‑1 (MQ=255)                  |                                                              GTTTTGCTTTCTCACCCCGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAGGGTTATTTTCGCCCGCATA  >  W3110S.gb/739663‑739741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑