Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	745,140	C→T	S159F (TCT→TTT)	ybgK →	predicted enzyme subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	745,140	0	C	T	100.0%	116.9 / NA	65	S159F (TCT→TTT)	ybgK	predicted enzyme subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/65);  total (0/65)

TGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTT  >  W3110S.gb/745105‑745159                                    |                    tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:70267/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:11670/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:5536/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:55897/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:56355/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:5711/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:58443/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:59095/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:59669/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:60285/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:62157/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:62485/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:65325/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:69104/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:69258/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:69437/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:54616/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:70315/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:73428/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:74247/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:74613/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:75198/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:75381/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:7724/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:77254/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:7806/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:79139/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:79433/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:86150/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:86646/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:87105/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:87138/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:31890/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:12876/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:12883/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:14842/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:15349/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:17444/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:18242/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:18425/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:20108/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:2380/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:23827/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:25061/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:27964/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:28228/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:31423/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:54002/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:41085/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:53702/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:53368/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:52866/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:51246/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:5076/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:50644/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:4436/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:43055/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:3284/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:39470/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:36121/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:36030/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:35364/55‑1 (MQ=255) tGACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:35274/55‑1 (MQ=255)  gACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATTGAAGCGCAGGGCGtt  <  1:73990/54‑1 (MQ=255)  gACCGACTCCCGATTGGCAAAGCGAAGCGTGATTTTATGGAAGCGCAGGGCGtt  <  1:73609/54‑1 (MQ=255)                                    |                    TGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTT  >  W3110S.gb/745105‑745159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑