Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	747,934	T→C	A86A (GCA→GCG)	abrB ←	predicted regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	747,934	0	T	C	100.0%	138.2 / NA	72	A86A (GCA→GCG)	abrB	predicted regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (72/0);  total (72/0)

ACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGAC  >  W3110S.gb/747895‑747957                                        |                        accCGCGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTg    >  1:62230/1‑61 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCa        >  1:50713/1‑57 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGtt     >  1:47108/1‑60 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTg    >  1:65765/1‑61 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTg    >  1:48471/1‑61 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTg    >  1:44510/1‑61 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTg    >  1:40192/1‑61 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTg    >  1:61342/1‑61 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTg    >  1:26053/1‑61 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:77356/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:10789/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:86394/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:49425/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:85018/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:51152/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:52328/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:82630/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:5318/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:54192/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:80884/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:57347/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:5801/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:59890/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:80261/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:76252/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:62397/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:6246/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:64581/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:86721/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:66293/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:66712/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:77260/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:67158/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:67213/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:67423/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:75402/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:75894/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:76017/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:19287/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:48466/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:268/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:25710/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:25472/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:24875/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:20909/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:20178/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:2014/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:29333/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:17328/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:16356/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:15652/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:12889/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:12184/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:11022/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:10844/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:39192/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:47021/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:46877/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:46732/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:44673/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:41726/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:40513/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:30054/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:362/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:35456/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:34248/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:33174/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:32550/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:32158/1‑62 (MQ=38) accCACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGa   >  1:30464/1‑62 (MQ=38)  cccACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGAc  >  1:54555/1‑62 (MQ=38)  cccACGATGGCGCTGGAAAGCAGCGTCACCAGTAAAATCGCAAGCACGATCGGCCAGTTGAc  >  1:12649/1‑62 (MQ=38)                                        |                        ACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGAC  >  W3110S.gb/747895‑747957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑