Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 66,476 | T→G | T25T (ACA→ACC) | araD ← | L‑ribulose‑5‑phosphate 4‑epimerase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 66,476 | 0 | T | G | 98.7% | 143.4 / ‑5.4 | 75 | T25T (ACA→ACC) | araD | L‑ribulose‑5‑phosphate 4‑epimerase |
Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (0/74); minor base C (0/1); total (0/75) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 |
GCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTG > W3110S.gb/66440‑66501 | gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:59176/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:6559/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:65300/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:6383/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:63114/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:62825/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:62779/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:62682/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:59984/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:67053/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:56004/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:54953/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:54149/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:53757/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:53377/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:51679/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:45885/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:42366/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:8082/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:9481/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:88165/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:8812/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:88111/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:87320/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:86780/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:85925/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:83732/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:43871/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:79301/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:78907/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:77906/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:75999/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:74854/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:73576/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:68159/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:19768/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:41995/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:25578/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:25569/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:24415/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:24051/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:22485/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:21914/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:21899/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:21165/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:25688/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:18100/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:15522/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:15326/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:13598/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:13083/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:11973/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:11640/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:11628/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:26597/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:4130/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:40339/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:3986/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:39664/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:37841/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:36135/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:35877/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:35712/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:35319/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:33606/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:29671/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:28795/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:2731/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:259/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGGGACCAGGTTGTGTTTTg < 1:8874/62‑1 (MQ=37) gccgcGCCCGCGATCAACGGCGCTGACATTGCCCCACGTGAGCGTGACCAGGTTGTGTTTTg < 1:28748/62‑1 (MQ=37) ccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:78794/61‑1 (MQ=37) ccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:55551/61‑1 (MQ=37) ccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:48898/61‑1 (MQ=37) ccgcGCCCGCGATCAACGGCGCTGACATTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTg < 1:11347/61‑1 (MQ=37) | GCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTG > W3110S.gb/66440‑66501 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |