Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	918,657	G→T	R296R (CGA→AGA)	ybjX ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	918,657	0	G	T	100.0%	101.1 / NA	55	R296R (CGA→AGA)	ybjX	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (55/0);  total (55/0)

CAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCGTGCTATCTGTGCTGGAA  >  W3110S.gb/918618‑918674                                        |                  cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGc       >  1:29125/1‑52 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:73006/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:50703/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:51234/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:51404/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:51628/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:53238/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:55107/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:55739/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:6151/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:63312/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:68143/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:69394/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:70779/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:71902/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:50581/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:73876/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:74528/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:75249/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:76124/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:76941/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:77487/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:78628/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:79711/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:80036/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:82427/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:88172/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:9989/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:33881/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:13609/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:13924/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:15869/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:18470/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:19828/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:2193/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:23858/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:23870/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:25220/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:30361/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:31077/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:3321/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:33442/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:11344/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:38603/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:39136/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:39702/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:41015/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:41036/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:45500/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:47216/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:47879/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:47885/1‑57 (MQ=255) cAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:49560/1‑57 (MQ=255) cAGCCCG‑TTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:37057/1‑56 (MQ=255) cAGACCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCTTGCTATCTGTGCTGGaa  >  1:51176/1‑57 (MQ=255)                                        |                  CAGCCCGTTTTTTACTGGCGATTTCGGCAATCTCTTTTCGTGCTATCTGTGCTGGAA  >  W3110S.gb/918618‑918674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑