Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	979,828	T→G	S1027S (TCT→TCG)	mukB →	fused chromosome partitioning proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	979,828	0	T	G	100.0%	186.3 / NA	99	S1027S (TCT→TCG)	mukB	fused chromosome partitioning proteins
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (99/0);  total (99/0)

GTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGAC  >  W3110S.gb/979773‑979834                                                        |       gccACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:27795/3‑62 (MQ=255) gacACGCAGCGCAGTTGACTCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:36691/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCCGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:24604/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACACCCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:75504/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTa      >  1:45530/3‑58 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:60826/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:65902/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:64609/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:64443/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:63596/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:63577/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:6343/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:63335/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:61931/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:61701/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:61540/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:61179/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:67681/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:57763/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:56522/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:55722/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:54007/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:5339/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:52860/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:51112/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:50003/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:49337/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:49161/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:72272/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:10079/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:87852/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:85628/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:83170/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:8121/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:80255/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:79749/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:79042/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:78614/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:7384/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:73033/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:66519/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:71395/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:71166/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:70907/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:70780/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:69987/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:69664/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:69386/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:69147/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:67724/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:67691/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:8842/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:17852/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:28781/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:27982/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:24178/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:23898/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:22670/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:21913/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:2103/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:20598/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:20493/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:19759/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:18701/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:2942/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:17737/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:17559/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:1731/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:16849/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:15749/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:14738/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:12946/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:11759/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:11663/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:10356/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:10177/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:35168/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:45875/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:43571/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:43286/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:43008/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:42416/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:39587/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:36980/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:36277/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:36212/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:36043/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:35270/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:4916/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:34637/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:34563/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:33633/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:32195/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:31235/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:3100/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:30705/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:30315/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:297/3‑62 (MQ=255) gacACGCAGCGCAGTTGAATCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCGTATGac  >  1:29531/3‑62 (MQ=255)                                                        |       GTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGAC  >  W3110S.gb/979773‑979834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑