Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	990,911	T→A	intergenic (‑133/‑133)	pncB ← / → pepN	nicotinate phosphoribosyltransferase/aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	990,911	0	T	A	100.0%	53.6 / NA	32	intergenic (‑133/‑133)	pncB/pepN	nicotinate phosphoribosyltransferase/aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (32/0);  total (32/0)

CATTACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGT  >  W3110S.gb/990908‑990949    |                                       cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:15172/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:86355/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:84229/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:77528/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:76598/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:73437/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:72511/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:69063/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:68678/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:68060/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:66746/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:65086/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:62861/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:62281/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:54556/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:51211/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:4720/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:40372/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:3873/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:3820/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:34930/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:25778/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:24440/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:23548/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:21911/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:21868/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:21814/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:198/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:18038/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:17356/1‑42 (MQ=255) cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt  >  1:12887/1‑42 (MQ=255) cATAACGCGGTGTAAGTTATAAAAATGTAGCAATaaa       >  1:48762/1‑37 (MQ=38)    |                                       CATTACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGT  >  W3110S.gb/990908‑990949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑