Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	992,516	T→C	D491D (GAT→GAC)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	992,516	0	T	C	100.0%	170.5 / NA	87	D491D (GAT→GAC)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (87/0);  total (87/0)

CATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGC  >  W3110S.gb/992487‑992541                              |                          cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCaaa               >  1:82560/1‑42 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTg            >  1:85828/1‑45 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGt     >  1:20564/1‑52 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTg   >  1:39831/1‑54 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:70358/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:70867/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:6963/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:68840/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:68818/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:6802/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:67900/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:67424/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:66280/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:62723/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:61547/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:59725/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:59631/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:58738/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:55747/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:52406/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:52362/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:5205/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:51671/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:80796/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:9361/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:916/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:87923/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:87201/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:86346/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:86267/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:84465/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:83691/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:8111/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:70704/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:80748/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:79818/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:76963/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:76643/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:74175/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:7314/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:72500/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:5133/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:70862/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:23375/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:11168/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:34448/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:34286/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:29075/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:29025/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:27319/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:27177/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:26637/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:25633/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:23444/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:35668/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:20356/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:19871/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:19423/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:15939/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:15800/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:1550/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:1316/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:12742/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:11668/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:43221/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:504/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:4964/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:49228/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:4733/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:46873/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:45930/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:45586/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:44568/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:4382/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:3630/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:42151/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:41311/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:41189/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:40509/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:40138/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:37534/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:37290/1‑55 (MQ=255) cATATTCCGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGCGATCCCGTTGc  >  1:15299/1‑55 (MQ=38) cATATTCCGTTTGCCATCGAACTGTAAGACAACGAAGGCAAAGTg            >  1:67098/1‑45 (MQ=38) cATATTCCGTTTGCAATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:85243/1‑55 (MQ=39) cATATTCAGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTGc  >  1:66170/1‑55 (MQ=39) cATATTACGTTTGCCATCGAACTGTATGACAACGAAGGCAAAGTGATCCCGTTg   >  1:43218/1‑54 (MQ=38)                              |                          CATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGC  >  W3110S.gb/992487‑992541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑