Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	140,332	T→A	R298R (CGA→CGT)	gcd ←	glucose dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	140,332	0	T	A	100.0%	134.1 / NA	71	R298R (CGA→CGT)	gcd	glucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/71);  total (0/71)

CGCAATCAGTCGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACT  >  W3110S.gb/140323‑140383          |                                                    tgCAATCAGACGACCATCATTGCCCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:53639/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGACTGATACGACGCGGGCAATCCGCCATCACt  <  1:80422/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACTCGGGCAATCCGCCATCACt  <  1:51536/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCTGGCAATCCGCCATCACt  <  1:29393/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:69291/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:70831/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:6921/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:67410/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:65712/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:64283/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:63746/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:61013/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:60151/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:55492/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:55244/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:54031/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:52991/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:52384/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:46550/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:80075/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:9783/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:9606/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:943/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:88629/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:8697/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:85126/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:833/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:83224/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:70088/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:79198/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:76462/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:75898/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:7589/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:72659/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:71898/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:12006/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:19776/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:29484/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:29118/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:28548/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:26866/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:25987/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:2329/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:22038/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:20119/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:31938/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:18734/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:18363/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:16408/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:14561/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:13877/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:13100/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:12643/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:10739/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:32174/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:44344/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:44106/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:43115/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:42284/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:41292/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:41006/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:39326/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:38785/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:37329/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:36262/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:34362/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:33324/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:33105/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:33041/60‑1 (MQ=255) tgCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:45473/60‑1 (MQ=255)  gCAATCAGACGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACt  <  1:44546/60‑1 (MQ=255)          |                                                    CGCAATCAGTCGACCATCATTGACCGGAAGAATGATACGACGCGGGCAATCCGCCATCACT  >  W3110S.gb/140323‑140383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑