Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,062,364	G→T	P48P (CCG→CCT)	torD →	chaperone involved in maturation of TorA subunit of trimethylamine N‑oxide reductase system I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,062,364	0	G	T	100.0%	48.6 / NA	29	P48P (CCG→CCT)	torD	chaperone involved in maturation of TorA subunit of trimethylamine N‑oxide reductase system I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/29);  total (0/29)

CTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTG  >  W3110S.gb/1062350‑1062404               |                                         cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:48771/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:85901/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:84442/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:83640/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:83467/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:78154/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:77343/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:72959/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:72269/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:60467/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:60332/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:56422/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:54939/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:49570/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:49406/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:13641/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:46059/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:45390/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:44656/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:43719/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:40008/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:33505/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:31722/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:25867/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:22750/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:21105/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:20389/55‑1 (MQ=255) cTGAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:19329/55‑1 (MQ=255)   gAAAAGCGAACCTCCGCTCACTGCGGCGGTGAATGGGCTGGAAAACAGTATTg  <  1:42422/53‑1 (MQ=255)               |                                         CTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTG  >  W3110S.gb/1062350‑1062404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑