Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,062,448	G→T	A76A (GCG→GCT)	torD →	chaperone involved in maturation of TorA subunit of trimethylamine N‑oxide reductase system I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,062,448	0	G	T	100.0%	101.9 / NA	59	A76A (GCG→GCT)	torD	chaperone involved in maturation of TorA subunit of trimethylamine N‑oxide reductase system I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/59);  total (0/59)

TATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGC  >  W3110S.gb/1062400‑1062457                                                 |          tATTGCGAATCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:52059/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:66279/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:11021/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:49175/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:49188/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:53481/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:55117/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:57151/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:57917/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:59093/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:59563/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:6037/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:60709/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:65142/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:65522/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:45808/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:70263/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:74267/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:74924/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:76244/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:77167/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:77950/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:85097/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:86911/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:87675/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:87800/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:87927/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:88445/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:88662/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:30204/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:11972/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:13131/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:14004/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:16588/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:16905/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:199/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:21379/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:21555/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:25724/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:29238/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:45761/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:30338/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:36517/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:4507/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:44994/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:40552/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:40068/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:38620/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:30676/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:35492/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:34722/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:34567/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:32008/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:3099/58‑1 (MQ=255) tATTGCCACTCTGACAGTACGTGACGATGCCCGTCAGGAACTGGCCGCTGACTTTTGc  <  1:5212/58‑1 (MQ=255)  aTTGCCACTCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:75788/57‑1 (MQ=255)                aGTACGTGACGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:29114/43‑1 (MQ=255)                        aCGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:20813/35‑1 (MQ=255)                        aCGATGCCCGTCTGGAACTGGCCGCTGACTTTTGc  <  1:12185/35‑1 (MQ=255)                                                 |          TATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGC  >  W3110S.gb/1062400‑1062457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑