Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,077,640	A→G	R555R (CGT→CGC)	putA ←	fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,077,640	0	A	G	100.0%	104.0 / NA	55	R555R (CGT→CGC)	putA	fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/55);  total (0/55)

TTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAA  >  W3110S.gb/1077627‑1077688              |                                                 tttCCAGCAGGCGGCTCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:22857/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGGAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:65237/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:73782/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:55865/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:57375/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:57999/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:60345/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:60509/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:61254/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:61322/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:63024/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:68794/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:70367/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:71823/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:7235/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:53175/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:74274/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:75639/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:75933/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:76397/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:76597/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:76899/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:77711/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:78501/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:78786/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:80576/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:84501/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:9886/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:12691/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:16047/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:16662/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:16973/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:17383/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:19984/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:27809/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:28725/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:33629/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:36271/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:36289/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:36719/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:4102/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:42123/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:42595/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:4450/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:46855/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:47066/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:48258/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:50718/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:50928/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:51468/62‑1 (MQ=255) tttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:53452/62‑1 (MQ=255) tttCCAGCAGGCAGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:2946/62‑1 (MQ=255) tttACAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:28916/62‑1 (MQ=255)  ttCCAGCAGGCGGCGCACCAGATATGCCAGCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:46105/61‑1 (MQ=255)  ttCCAGCAGGCGGCGCACCAGATATGCCACCAGCGTTTCATGTGTACCGACCGGAGCATaa  <  1:14731/61‑1 (MQ=255)              |                                                 TTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGTGCCAACCGGAGCATAA  >  W3110S.gb/1077627‑1077688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑