Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	140,707	A→G	R173R (CGT→CGC)	gcd ←	glucose dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	140,707	0	A	G	100.0%	54.4 / NA	30	R173R (CGT→CGC)	gcd	glucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/30);  total (0/30)

CGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCC  >  W3110S.gb/140683‑140723                         |                 tgAAAATCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:20165/40‑1 (MQ=38) tgAAAAGCGTTGACCTTCCTGATTGCGTCCATAGGCAGGcc  <  1:37706/40‑1 (MQ=38) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:5597/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:84098/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:83892/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:81861/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:80769/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:7928/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:71334/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:71215/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:70524/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:70248/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:68317/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:63509/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:63457/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:14550/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:48366/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:42714/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:41143/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:41027/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:40233/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:37004/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:30595/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:25717/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:19626/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:19204/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:19028/40‑1 (MQ=39) tgAAAAGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:1749/40‑1 (MQ=39)   aaaaGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:57583/39‑1 (MQ=255)     aaGCGTTGACCTTCCTGATTGCGACCATAGGCAGGcc  <  1:77398/37‑1 (MQ=255)                         |                 CGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCC  >  W3110S.gb/140683‑140723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑