Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,142,368	G→A	Q253Q (CAG→CAA)	flgL →	flagellar hook‑filament junction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,142,368	0	G	A	100.0%	131.3 / NA	73	Q253Q (CAG→CAA)	flgL	flagellar hook‑filament junction protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (73/0);  total (73/0)

CGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTT  >  W3110S.gb/1142364‑1142415     |                                                cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGt                >  1:802/1‑38 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGt                >  1:58552/1‑38 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTa               >  1:7794/1‑39 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTa               >  1:66505/1‑39 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTa               >  1:57282/1‑39 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTa               >  1:15834/1‑39 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGc             >  1:21005/1‑41 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCg            >  1:38839/1‑42 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGa           >  1:9629/1‑43 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGa           >  1:18142/1‑43 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGCTCGCGCttt  >  1:75845/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:57430/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:61211/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:56615/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:65354/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:55460/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:73085/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:53080/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:74310/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:45577/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:74382/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:75828/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:51804/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:76112/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:76640/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:78952/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:80282/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:80406/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:81287/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:8153/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:81925/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:83872/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:83955/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:87246/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:87526/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:88223/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:9837/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:9850/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:18516/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:51362/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:27735/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:25436/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:24181/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:23498/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:22053/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:21397/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:20286/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:28115/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:16835/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:14714/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:14161/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:13545/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:12034/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:1061/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:10473/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:38617/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:49076/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:1014/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:42305/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:41173/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:40319/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:39525/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:3926/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:28307/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:38519/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:36228/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:34106/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:32925/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:32715/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCttt  >  1:31389/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCgtt  >  1:71872/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGAGATCGCGCttt  >  1:2748/1‑52 (MQ=255) cGCAACTGAACGAACTGGAGTCGCTGGATACATTAGGTAGCGATCGCGCttt  >  1:13301/1‑52 (MQ=255)     |                                                CGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTT  >  W3110S.gb/1142364‑1142415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑