Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	147,953	C→T	E281E (GAG→GAA)	panC ←	pantothenate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	147,953	0	C	T	100.0%	129.0 / NA	73	E281E (GAG→GAA)	panC	pantothenate synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/73);  total (0/73)

TAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTC  >  W3110S.gb/147909‑147970                                             |                  taaCTGGCGCCATCAGCTGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:62520/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:5933/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:7362/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:73603/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:73519/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:69884/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:68891/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:63422/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:63338/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:73907/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:57176/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:54421/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:50195/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:49123/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:10528/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:48717/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:46717/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:4904/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:74148/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:7424/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:74841/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:76153/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:78944/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:79064/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:79105/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:80143/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:81841/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:82549/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:82788/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:8283/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:83858/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:84287/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:88635/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:14369/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:19044/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:18609/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:20681/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:20952/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:21661/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:22479/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:24332/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:25468/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:43439/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:26934/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:17717/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:28273/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:15391/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:28478/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:29258/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:30660/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:31876/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:32399/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:32726/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:14334/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:34692/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:11103/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:3742/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:39272/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:40243/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:43129/62‑1 (MQ=255) taaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:10589/62‑1 (MQ=255) taaCTGGCGCCACCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:2636/62‑1 (MQ=255)  aaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:79188/61‑1 (MQ=255)  aaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:20452/61‑1 (MQ=255)  aaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:81454/61‑1 (MQ=255)  aaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:69853/61‑1 (MQ=255)  aaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:64805/61‑1 (MQ=255)  aaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:50984/61‑1 (MQ=255)  aaCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:88179/61‑1 (MQ=255)   aCTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:12537/60‑1 (MQ=255)     tGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:27614/58‑1 (MQ=255)           cATCAGCAGTAGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:57017/52‑1 (MQ=255)                     aGCGCCAGTTAAGTATTACGCCAGTTCGACCATTTTGTTGTc  <  1:48310/42‑1 (MQ=255)                                             |                  TAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGACCATTTTGTTGTC  >  W3110S.gb/147909‑147970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑