Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,176,578 G→T A55A (GCC→GCA)  ycfT ← predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,176,5780GT100.0% 205.9 / NA 109A55A (GCC→GCA) ycfTpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (109/0);  total (109/0)

Only 100 of 109 total aligned reads displayed.

TACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAA  >  W3110S.gb/1176568‑1176628
          |                                                  
tACGAAAGGGTGCAAGGTAAAGATTGAAATATATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:24679/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCa                       >  1:19150/1‑40 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGTTCAGGACTTCTGATaa  >  1:81924/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTc                >  1:85863/1‑47 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTct       >  1:66769/1‑55 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTc        >  1:31204/1‑55 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:60259/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:6939/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:67462/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:67089/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:66289/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:66139/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:65429/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:65295/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:62328/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:61621/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:60455/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:7191/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:60225/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:57740/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:57706/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:56230/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:55292/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:55079/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:53548/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:5311/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:52548/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:50202/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:78702/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:9335/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:88332/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:86239/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:86005/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:85820/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:85706/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:8312/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:82772/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:82492/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:82262/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:79201/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:71190/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:77921/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:77642/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:76586/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:7635/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:75341/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:74627/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:73318/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:72875/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:4883/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:71559/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:19770/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:30906/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:30854/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:30348/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:2824/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:25996/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:2581/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:25/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:24987/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:24342/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:23530/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:21241/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:31919/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:19369/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:18364/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:18220/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:18129/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:17514/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:16012/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:16011/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:15092/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:14229/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:12819/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:11444/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:49704/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:4780/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:47710/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:46110/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:45945/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:45787/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:44920/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:44751/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:43735/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:4362/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:42865/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:41912/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:41879/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:41560/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:39924/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:38709/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:37902/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:36893/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:36888/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:34614/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:34495/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:34154/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATaa  >  1:11157/1‑61 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATa   >  1:6063/1‑60 (MQ=255)
tACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCTGATa   >  1:31141/1‑60 (MQ=255)
          |                                                  
TACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAA  >  W3110S.gb/1176568‑1176628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑