Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,177,463	C→T	L154M (CTA→TTG)	lolC →	outer membrane‑specific lipoprotein transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,177,463	0	C	T	98.4%	115.9 / NA	63	L154M (CTA→TTG)	lolC	outer membrane‑specific lipoprotein transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (62/0);  minor base A (1/0);  total (63/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCG  >  W3110S.gb/1177417‑1177478                                               |                gCCGGGGAAATATAATGTCATTCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:1962/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGATCAGCTTGCCTCACAGATGGGCGTCAATc     >  1:49316/1‑59 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGtc         >  1:55069/1‑54 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:65544/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:39353/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:41307/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:42835/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:44048/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:54690/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:55023/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:56448/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:56916/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:61843/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:62094/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:63340/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:64086/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:64906/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:38665/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:65678/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:65944/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:69918/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:71705/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:77549/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:77990/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:78094/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:83869/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:8458/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:85694/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:85875/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:85952/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:88282/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:26438/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:11366/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:11708/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:12635/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:13492/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:14338/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:14795/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:1552/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:18557/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:19774/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:20914/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:2196/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:26126/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:1117/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:27549/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:3038/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:37574/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:3714/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:37/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:36314/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:3586/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:34661/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:34174/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:32598/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:32204/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:3136/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgcg  >  1:30329/1‑62 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgc   >  1:17502/1‑61 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgc   >  1:48814/1‑61 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcgag  >  1:49357/1‑60 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATcg    >  1:16253/1‑60 (MQ=255) gCCGGGGAAATATAATGTCATCCTCGGTGAACAGCTTGCCTCACAGTTGGGCGTCAATc     >  1:26286/1‑59 (MQ=255)                                               |                GCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCG  >  W3110S.gb/1177417‑1177478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑