Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,227,488	T→G	D57A (GAC→GCC)	minC ←	cell division inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,227,488	0	T	G	100.0%	27.6 / NA	17	D57A (GAC→GCC)	minC	cell division inhibitor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (0/17);  total (0/17)

TACCGGGTCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCGG  >  W3110S.gb/1227481‑1227539        |                                                    tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:36994/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:87024/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:86036/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:78691/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:70927/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:60432/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:55228/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:47345/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:43119/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:11593/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:32272/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:2342/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:21581/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:18169/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:14448/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:12382/59‑1 (MQ=255) tACCGGGGCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCgg  <  1:12116/59‑1 (MQ=255)        |                                                    TACCGGGTCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCGG  >  W3110S.gb/1227481‑1227539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑