Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,346,666	C→T	Q597Q (CAG→CAA)	gmr ←	modulator of Rnase II stability

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,346,666	0	C	T	100.0%	75.2 / NA	42	Q597Q (CAG→CAA)	gmr	modulator of Rnase II stability
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/0);  total (42/0)

CGACGATCGCCCGGACCAGTGACTGCGAGACAGGTTGTTTGTGAATATCTCGAACAAAAACC  >  W3110S.gb/1346644‑1346705                       |                                        cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:70619/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:4440/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:4969/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:50257/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:53458/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:55145/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:55633/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:59790/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:61996/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:67398/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:68814/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:43535/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:71576/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:75584/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:76860/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:77136/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:77323/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:78538/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:81960/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:83460/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:86838/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:27855/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:13553/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:17941/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:19553/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:19563/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:19890/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:20405/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:20672/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:21527/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:25273/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:26291/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:12032/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:28420/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:29759/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:30227/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:30663/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:31400/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:35084/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:35417/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:37199/1‑62 (MQ=255) cgacgaTCGCCCGGACCAGTGATTGCGAGACAGGCTGTTTGTGAATATCCCGAACAAAAAcc  >  1:38275/1‑62 (MQ=255)                       |                                        CGACGATCGCCCGGACCAGTGACTGCGAGACAGGTTGTTTGTGAATATCTCGAACAAAAACC  >  W3110S.gb/1346644‑1346705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑