Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,361,733	G→A	S297F (TCT→TTT)	puuA ←	gamma‑Glu‑putrescine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,361,733	0	G	A	100.0%	52.4 / NA	31	S297F (TCT→TTT)	puuA	gamma‑Glu‑putrescine synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/0);  total (31/0)

TCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACG  >  W3110S.gb/1361680‑1361741                                                      |         tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGccca  >  1:55072/1‑59 (MQ=38) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:62412/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:9483/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:83796/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:81738/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:81235/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:79137/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:77717/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:77227/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:75359/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:75220/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:7194/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:70863/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:66022/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:6386/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:63582/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:13672/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:60178/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:59983/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:58640/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:49914/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:46366/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:39262/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:36757/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:36641/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:303/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:2906/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:26007/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:16372/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAca  >  1:13915/1‑61 (MQ=39) tcatcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAAAGAGCAc   >  1:7266/1‑61 (MQ=255)                                                      |         TCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACG  >  W3110S.gb/1361680‑1361741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑