Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,442,292	G→A	G69G (GGC→GGT)	ydbK ←	fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,442,292	0	G	A	100.0%	142.7 / NA	79	G69G (GGC→GGT)	ydbK	fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (79/0);  total (79/0)

GCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGT  >  W3110S.gb/1442285‑1442343        |                                                    gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGCCTGCATTTCAACCAcgcg   >  1:11115/1‑58 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTg                  >  1:53375/1‑43 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAcgcgc  >  1:23583/1‑58 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAcgcg   >  1:60188/1‑58 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCAcgcg   >  1:18163/1‑58 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:65213/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:7072/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:64009/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:63937/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:63936/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:63912/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:63841/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:6327/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:62960/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:62493/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:6045/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:58598/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:55916/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:55704/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:5570/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:55008/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:54452/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:77293/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:87612/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:87124/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:83414/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:82136/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:81127/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:79754/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:78476/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:77668/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:77476/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:68354/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:75622/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:75508/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:7547/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:74997/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:74587/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:71552/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:5129/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:6865/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:20957/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:37901/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:37274/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:35929/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:34905/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:33074/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:32206/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:31965/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:3004/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:22921/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:38168/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:16930/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:16910/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:13528/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:12846/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:11829/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:1164/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:11304/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:10945/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:45053/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:51641/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:10727/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:4932/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:49206/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:47302/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:46221/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:46145/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:45828/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:52539/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:4502/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:41837/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:40219/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:40080/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:4/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:39996/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:39963/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCgt  >  1:39858/1‑59 (MQ=255) gCAAAGCACCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACAACGCgt  >  1:50808/1‑59 (MQ=255)        |                                                    GCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCAACCACGCGT  >  W3110S.gb/1442285‑1442343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑