Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,513,069	G→A	E451E (GAG→GAA)	ydcR →	fused predicted DNA‑binding transcriptional regulator and predicted amino transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,513,069	0	G	A	100.0%	94.7 / NA	52	E451E (GAG→GAA)	ydcR	fused predicted DNA‑binding transcriptional regulator and predicted amino transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/52);  total (0/52)

TACCGCGTGGCAGTGGGGAGAGCGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTATT  >  W3110S.gb/1513048‑1513108                      |                                        tACCGCGTGGCGGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:49847/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCTGTAAAACAATTAGGCAAACTTAtt  <  1:9284/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:41333/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:54157/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:10968/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:56833/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:5842/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:59389/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:616/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:63998/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:6673/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:70384/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:70929/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:77243/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:7777/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:81269/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:81402/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:83561/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:87029/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:21296/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:12469/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:14652/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:15188/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:18048/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:18796/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:22319/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:26222/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:30008/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:30793/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:38885/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:35272/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:37719/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:33447/61‑1 (MQ=255) tACCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGCAAAACAATTAGGCAAACTTAtt  <  1:39172/61‑1 (MQ=255) tACCGCGTCGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:82505/61‑1 (MQ=255)  aCCGCGTGGCAGTGGTGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:3709/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:78863/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:87710/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:44007/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:86535/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:85213/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:45046/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:82295/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:81944/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:17466/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:48412/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:1846/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:75211/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:71474/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:50733/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:27611/60‑1 (MQ=255)  aCCGCGTGGCAGTGGGGAGAACGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTAtt  <  1:60051/60‑1 (MQ=255)                      |                                        TACCGCGTGGCAGTGGGGAGAGCGTGAAGAACAGGCGGTAAAACAATTAGGCAAACTTATT  >  W3110S.gb/1513048‑1513108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑