Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,679,093 A→G V175V (GTT→GTC)  pntA ← pyridine nucleotide transhydrogenase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,679,0930AG98.5% 125.7 / ‑5.6 66V175V (GTT→GTC) pntApyridine nucleotide transhydrogenase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (0/65);  minor base T (0/1);  total (0/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CAGACCTGCAACACCCGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAG  >  W3110S.gb/1679084‑1679145
         |                                                    
cAGACCTGCTACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:55220/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:66266/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:72469/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:72195/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:71502/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:7098/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:69651/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:67783/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:67058/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:66935/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:73034/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:65117/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:64348/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:64227/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:64031/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:6273/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:60578/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:80112/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:984/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:88533/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:87388/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:87326/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:86606/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:81728/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:81371/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:8084/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:60440/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:79528/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:78592/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:78021/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:77848/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:77118/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:7679/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:73984/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:3844/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:12591/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:14789/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:18325/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:22314/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:23276/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:24349/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:25394/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:26270/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:28573/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:3033/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:32642/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:33626/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:36959/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:37101/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:1111/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:3990/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:41030/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:45404/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:46318/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:48076/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:48886/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:50348/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:51695/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:5278/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:56486/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:57900/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:59102/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:59671/62‑1 (MQ=255)
cAGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:60138/62‑1 (MQ=255)
 aGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCTGTGGCACTTTCCCGGCCGCAg  <  1:46350/61‑1 (MQ=38)
 aGACCTGCGACACCGGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAg  <  1:32556/61‑1 (MQ=255)
         |                                                    
CAGACCTGCAACACCCGCACCAATCACCATCACTTTTGCCGGTGGCACTTTCCCGGCCGCAG  >  W3110S.gb/1679084‑1679145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑