Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,738,861	G→A	A48A (GCC→GCT)	ydhP ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,738,861	0	G	A	100.0%	159.2 / NA	88	A48A (GCC→GCT)	ydhP	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (88/0);  total (88/0)

GCCAACTGCATAGGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACCGCGC  >  W3110S.gb/1738849‑1738909             |                                                 gCCAACTGCATAAGCACTGATTAACATTCCGTCAGCGGGAATCGAGACATCCACACcgcgc  >  1:60442/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGc                           >  1:28070/1‑36 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGCCATCCACACcgcgc  >  1:61147/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATTCACACcgcgc  >  1:46734/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCcac         >  1:73294/1‑54 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:64676/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:71431/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:71426/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:70897/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:69278/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:69110/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:68448/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:67180/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:66824/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:65599/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:58678/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:63162/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:61985/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:61249/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:61174/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:61114/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:59475/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:59126/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:54357/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:80052/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:9457/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:88668/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:88386/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:87292/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:84958/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:84011/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:82724/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:8253/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:8071/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:8011/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:73804/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:79483/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:78751/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:7611/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:7511/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:7475/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:74679/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:74676/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:74210/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:74209/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:20179/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:280/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:27374/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:26317/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:26061/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:25676/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:25290/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:25080/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:24824/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:21952/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:11215/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:19869/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:18628/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:18065/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:17788/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:17416/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:17332/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:17/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:1600/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:14001/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:31990/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:58301/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:54253/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:54204/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:52999/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:52373/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:5103/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:49650/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:48274/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:47308/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:45767/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:45573/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:42322/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:3527/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:35198/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:3421/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:32846/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcgc  >  1:30167/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcg   >  1:26758/1‑60 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACcgcg   >  1:13586/1‑60 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGGAATCGAGACAACCACACcgcgc  >  1:15064/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCGGAAATCGAGACCTCCCCACcgcgc  >  1:33032/1‑61 (MQ=255) gCCAACTGCATAAGCACTGATTAACATTCCGGCAGCAGGAATCGAGACATCCACACcgcgc  >  1:49657/1‑61 (MQ=255)             |                                                 GCCAACTGCATAGGCACTGATTAACATTCCGGCAGCGGGAATCGAGACATCCACACCGCGC  >  W3110S.gb/1738849‑1738909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑