Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,796,360	T→A	S169S (TCA→TCT)	btuC ←	vitamin B12 transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,796,360	0	T	A	100.0%	139.4 / NA	77	S169S (TCA→TCT)	btuC	vitamin B12 transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (77/0);  total (77/0)

CTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGC  >  W3110S.gb/1796345‑1796397                |                                      cTGACGTAAATCAACAGAGGTTGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:36359/1‑53 (MQ=38) cTGACGTAAATCAACAGAGGTGGAAAAGTATATAGCCAACGTCa           >  1:27822/1‑44 (MQ=25) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGc                   >  1:43049/1‑36 (MQ=37) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:59474/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:6873/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:66755/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:64383/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:63634/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:62432/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:61294/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:59558/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:53948/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:59149/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:58965/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:58257/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:58086/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:56043/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:54544/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:54144/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:54040/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:12880/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:78468/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:9692/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:9647/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:88626/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:88621/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:87119/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:85147/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:82355/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:80681/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:80268/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:69198/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:77223/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:76318/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:75060/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:75000/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:74865/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:73535/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:71212/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:70097/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:22761/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:35709/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:33014/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:31840/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:2944/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:29374/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:25661/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:25518/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:23001/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:22888/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:36856/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:22631/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:22470/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:19620/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:14829/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:14696/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:13735/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:12985/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:1252/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:532/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:51360/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:51159/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:39575/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:40027/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:40546/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:41049/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:41793/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:43553/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:46341/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:48475/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:49302/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:49350/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:50663/1‑53 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcg   >  1:51499/1‑52 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcg   >  1:38545/1‑52 (MQ=255) cTGACGTAAATCAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGAgccc  >  1:36981/1‑51 (MQ=255) cTGACGTAAATAAACAGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTgcgc  >  1:66507/1‑53 (MQ=255)                |                                      CTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGC  >  W3110S.gb/1796345‑1796397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑