Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,837,990	C→T	V68V (GTC→GTT)	ynjB →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,837,990	0	C	T	100.0%	147.6 / NA	79	V68V (GTC→GTT)	ynjB	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (79/0);  total (79/0)

CATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCG  >  W3110S.gb/1837964‑1838006                           |                 cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCgg        >  1:45448/1‑37 (MQ=37) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGa       >  1:31928/1‑38 (MQ=37) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGAt      >  1:19619/1‑39 (MQ=37) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATg     >  1:66278/1‑40 (MQ=37) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGcc   >  1:16759/1‑42 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGc    >  1:6041/1‑41 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:71196/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:76529/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:69359/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:68263/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:66113/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:64911/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:64327/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:62369/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:60469/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:6034/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:60179/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:59145/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:58499/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:58311/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:57607/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:56699/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:8291/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:9017/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:88372/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:87306/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:8614/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:85561/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:85388/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:85244/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:84975/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:83476/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:733/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:81358/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:79909/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:79065/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:78950/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:78179/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:77588/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:54298/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:74731/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:26583/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:36460/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:35395/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:35064/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:30997/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:30286/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:29136/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:2839/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:28252/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:5441/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:25992/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:25894/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:19587/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:19015/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:1363/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:12392/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:11494/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:42896/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:55310/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:10991/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:52736/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:47109/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:45366/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:4373/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:43495/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:37416/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:4222/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:41700/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:41463/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:4077/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:40083/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGCCg  >  1:38628/1‑43 (MQ=38) cATTACGCTATAAACCTGAAGATTGTTCGTCTCGCGGATGACg  >  1:36697/1‑43 (MQ=37) cATTACGCTATAAACCTGAAGATTGTTCGTCACGCGGATGCCg  >  1:73311/1‑43 (MQ=37) cATTACGCTATAAACCTGAAGATTGTTAGTCTCGc          >  1:38333/1‑35 (MQ=21) cATTACGCTATAAACCTGAACATTGTTCGTCTCGCGGATGCCg  >  1:24018/1‑43 (MQ=37) cATTACGCTATAAACATGAAGATTGTTCGTCTCGCGGATGCCg  >  1:44067/1‑43 (MQ=255) cATTACGCTATAAACATGAAGATTGTTCGTCTCGCGGATGCCg  >  1:25816/1‑43 (MQ=255)                           |                 CATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCG  >  W3110S.gb/1837964‑1838006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑