Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 182,263 C→G L460L (CTC→CTG)  degP → serine endoprotease (protease Do), membrane‑associated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb182,2630CG100.0% 153.1 / NA 78L460L (CTC→CTG) degPserine endoprotease (protease Do), membrane‑associated
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (0/78);  total (0/78)

AACATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTCAACATTCA  >  W3110S.gb/182210‑182271
                                                     |        
aacaacGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:80454/62‑1 (MQ=255)
aaCGTCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:47884/62‑1 (MQ=255)
aaCATCGCTGAACTGGGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:84722/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAATCGTCTGTGCTGGCACTGAACATTCa  <  1:32415/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:58374/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:70993/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:69695/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:6913/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:67105/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:6466/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:61284/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:59971/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:59723/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:58655/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:71454/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:58354/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:56116/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:56031/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:54557/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:54451/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:5438/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:53549/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:79978/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:9809/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:87861/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:84844/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:83687/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:83265/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:82650/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:81891/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:81491/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:71087/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:78647/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:74809/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:74797/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:74724/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:73917/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:73639/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:72806/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:49812/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:19741/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:36226/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:33820/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:33649/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:3044/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:29010/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:26799/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:24171/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:23829/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:20569/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:36299/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:16683/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:165/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:14813/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:14343/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:14322/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:13191/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:12996/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:11383/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:406/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:11132/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:46156/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:45540/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:43836/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:43784/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:43187/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:42425/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:41695/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:40755/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:5227/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:40045/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:39837/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:38602/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:38392/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:3770/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:36906/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:36333/62‑1 (MQ=255)
aaCATCGCTGAACTGCGTAAAGTCCTCGACAGCAAACCGTCTGTGCTGGCACTGAACATTCa  <  1:38023/62‑1 (MQ=255)
                                                     |        
AACATCGCTGAACTGCGTAAAGTTCTCGACAGCAAACCGTCTGTGCTGGCACTCAACATTCA  >  W3110S.gb/182210‑182271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑