Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,866,100	G→A	G179G (GGG→GGA)	yeaD →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,866,100	0	G	A	100.0%	108.9 / NA	61	G179G (GGG→GGA)	yeaD	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (61/0);  total (61/0)

TAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAA  >  W3110S.gb/1866082‑1866143                   |                                            tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATaa                      >  1:242/1‑42 (MQ=38) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTaaa                >  1:33970/1‑48 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAGATGACGCgaaagaag  >  1:50436/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGa             >  1:37406/1‑51 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:72015/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:47837/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:48683/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:52230/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:53963/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:54475/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:54717/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:55445/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:60080/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:6242/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:62599/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:67038/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:70944/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:45920/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:75748/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:75860/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:76940/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:78281/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:80739/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:81904/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:82874/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:8320/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:84289/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:84962/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:86181/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:86753/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:9238/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:10448/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:11500/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:13450/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:16724/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:17588/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:18235/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:19259/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:19709/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:20070/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:20835/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:21805/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:22473/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:2311/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:24228/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:25255/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:32706/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:35756/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:3922/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:39915/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:41612/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:42057/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:42655/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:43171/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:45250/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:4559/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:45768/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaag  >  1:46980/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaa   >  1:24855/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaa   >  1:83945/1‑61 (MQ=255) tAAGGTAAGCGTCAGTGGACTAGGCGATCGCTTCATTGATAAAGTAAATGACGCgaaagaa   >  1:50184/1‑61 (MQ=255)                   |                                            TAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAA  >  W3110S.gb/1866082‑1866143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑