Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,890,138	T→C	V441V (GTA→GTG)	fadD ←	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,890,138	0	T	C	100.0%	115.2 / NA	61	V441V (GTA→GTG)	fadD	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (61/0);  total (61/0)

GAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATG  >  W3110S.gb/1890086‑1890147                                                     |          gAATCATGTCTTTTTTACTATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:35710/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGAt   >  1:86609/1‑61 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGAt   >  1:20206/1‑61 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGAt   >  1:42709/1‑61 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGAt   >  1:7883/1‑61 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGAt   >  1:28046/1‑61 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:64056/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:67411/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:63921/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:60507/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:60498/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:59847/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:56566/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:55537/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:54214/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:54151/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:48358/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:47488/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:64590/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:66029/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:46567/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:67720/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:68067/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:69218/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:71629/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:75036/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:80185/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:80923/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:8100/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:82521/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:83480/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:88658/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:30455/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:14978/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:14985/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:16288/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:17131/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:18028/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:18087/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:22040/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:23273/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:25494/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:26409/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:2975/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:29766/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:30314/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:47261/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:32320/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:35360/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:3823/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:39349/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:39406/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:42245/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:44611/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:4528/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:46450/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:13541/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACCGCGATg  >  1:47003/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATCACAGCGATg  >  1:31111/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATACATCACCGCGATg  >  1:32652/1‑62 (MQ=255) gAATCATGTCTTTTTTACGATCGACAATGCGAAGGAATCCTTCTTCATCCATCACCGCGa    >  1:82654/1‑60 (MQ=255)                                                     |          GAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATG  >  W3110S.gb/1890086‑1890147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑