Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,997,113	G→A	D128D (GAC→GAT)	uvrY ←	DNA‑binding response regulator in two‑component regulatory system with BarA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,997,113	0	G	A	100.0%	26.3 / NA	17	D128D (GAC→GAT)	uvrY	DNA‑binding response regulator in two‑component regulatory system with BarA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/17);  total (0/17)

TGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGA  >  W3110S.gb/1997077‑1997118                                     |      tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:64754/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:88229/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:86387/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:83428/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:75514/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:71746/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:71695/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:71265/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:66486/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:19968/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:55837/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:54179/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:49462/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:23592/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:22007/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:20845/42‑1 (MQ=255) tGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATATCAGa  <  1:20042/42‑1 (MQ=255)                                     |      TGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGA  >  W3110S.gb/1997077‑1997118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑