Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,000,485	A→G	R44R (CGT→CGC)	yecS ←	predicted transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,000,485	0	A	G	100.0%	82.9 / NA	46	R44R (CGT→CGC)	yecS	predicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/0);  total (46/0)

GCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTT  >  W3110S.gb/2000451‑2000546                                   |                                                              gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCa                                                           >  1:76331/1‑39 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATc                                                         >  1:45340/1‑41 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:66332/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:39002/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:39227/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:40041/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:40323/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:41670/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:42495/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:47089/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:47764/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:50008/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:57259/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:60365/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:61363/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:65214/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:12571/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:68547/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:69944/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:7008/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:73711/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:75591/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:79230/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:80061/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:81403/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:84579/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:8671/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:87445/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:9343/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:12470/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:21326/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:24166/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:24192/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:20760/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:20477/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:18984/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:18197/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:25458/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:16885/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:16572/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:25754/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:26314/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:13739/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:35093/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:32439/1‑42 (MQ=255) gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGGCGCATCa                                                        >  1:32047/1‑42 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:75904/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:63987/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:69135/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:87803/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:87681/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:21321/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:72234/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:74815/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:84652/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:12925/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:77629/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:78141/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:86777/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:86244/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:16503/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:84100/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:15165/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:86106/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:54668/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:36368/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:37715/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:38121/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:31122/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:30330/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:29928/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:28874/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:45372/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:2637/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:50347/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:53005/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:53999/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:67304/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:56931/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:57565/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:57901/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:58315/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:58344/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:58522/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:60083/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:25225/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:24694/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:61676/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:36328/62‑1 (MQ=255)                                   aCGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt  <  1:66878/62‑1 (MQ=255)                                   |                                                              GCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTT  >  W3110S.gb/2000451‑2000546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑