Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,024,368	A→C	I121I (ATA→ATC)	fliP →	flagellar biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,024,368	0	A	C	100.0%	159.4 / NA	82	I121I (ATA→ATC)	fliP	flagellar biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (82/0);  total (82/0)

CCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGC  >  W3110S.gb/2024348‑2024408                     |                                         ccATTCAGCGAAGTGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:4843/1‑61 (MQ=255) ccATTCAGCGAAGGGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:34372/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAgggg              >  1:13638/1‑49 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGgc             >  1:2649/1‑50 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCa          >  1:38589/1‑53 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTg   >  1:10552/1‑60 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTg   >  1:7740/1‑60 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:50670/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:50490/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:64315/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:60293/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:60145/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:86189/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:56653/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:56239/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:5571/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:5113/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:50894/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:55462/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:5496/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:5475/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:54313/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:54246/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:51938/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:78517/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:10414/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:85214/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:84789/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:83055/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:80773/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:80659/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:80562/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:79577/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:790/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:65764/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:77712/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:7702/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:74288/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:73729/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:7364/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:72716/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:70760/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:70681/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:69829/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:22207/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:50072/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:29496/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:2936/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:29179/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:28633/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:28600/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:24368/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:2264/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:22603/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:29532/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:2064/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:20035/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:19621/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:18760/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:1590/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:14899/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:12862/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:12692/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:39515/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:48236/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:47379/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:47224/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:4691/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:458/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:45778/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:43370/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:41077/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:39628/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:30830/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:39320/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:38738/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:37015/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:36285/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:34392/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:3417/1‑61 (MQ=255) ccATTCAGCGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCACAGCCGCTGc  >  1:56663/1‑61 (MQ=255) ccATTCAGAGAAGAGAAAATCTCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGc  >  1:28866/1‑61 (MQ=255)                     |                                         CCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGC  >  W3110S.gb/2024348‑2024408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑